ASTM E2149 PDF

ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.

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This test method is designed to evaluate the antimicrobial activity of non-leaching, antimicrobial-treated specimens under dynamic contact conditions. This test method is designed to evaluate the resistance of specimens treated with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions. Work Item s – proposed revisions of this standard.

All astn samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period. For example, water soluble e21449 will be prone to removal from the test surface using the method described in Section 13 but insoluble compounds will not. The presence of a leaching antimicrobial is determined post-test.

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Following exposure, a sample of the test organism suspension is removed quantitatively assayed for survivors. One-hour contact time in a buffer solution allows for metabolic stasis in the population. Link to Active This link will always route to the current Active version of the standard. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent.

It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.

ASTM E2149 Antimicrobial Activity

Organisms typically used in this method: Log in Log out Edit. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required. This dynamic shake aastm test was developed for routine quality control and screening test.

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This website stores cookies on your computer. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.

The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.

Summary of Test Method: The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. Facebook Linkedin Instagram Twitter Google. Link to Active This link will always route to the current Active version of the standard.

It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour.

Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration.

The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. Microbial concentrations in the treated and untreated samples are usually determined at 0, 1 or 24 hours contact time.

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ASTM E – Microbe Investigations (MIS)

The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent. Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.

This standard does not purport to address all of the safety concerns, if any, associated with its use. To find out more about the cookies we use, see our Privacy Policy.

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Work Item s – proposed revisions of this standard. It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.

The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension. No regulated reduction limits currently exist for general antimicrobial claims made using this method. Historical Version s – view previous versions of standard. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.

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This standard does not purport to address all of the safety concerns, if any, associated with its use. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part sstm the standard. No other units of measurement are included in this standard. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.

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