DEVELOPMENT OF AN AUTOMATED IMMUNOTURBIDIMETRIC FERRITIN ASSAY PDF

Such assays are suitable for the measurement of proteins at concentrations >5– 10 mg/L. .. Development of an automated immunoturbidimetric ferritin assay. May 3, Different laboratory methods are used to quantify ferritin . assays and automated equipment developments, and in routine laboratory assays and Seco M. Development of an automated immunoturbidimetric ferritin assay. J. Colloid. Interface Sci. (2): – 3. Borque, L., Rus, A., Bellod, L., and Seco, M.L. Development of an automated immunoturbidimetric ferritin assay.

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In this way, the inactive IgG molecules are replaced with molecules of BSA, remarkably increasing the latex stability by electrostatic repulsion. Skip to main content. The procedure has been automated using the Falcor analyzer.

A microparticle size of nm and a wavelength of nm were selected as the best choice because of the high slope and wide dynamic range showed. The turbidimetric method affords a measuring range of 0. Samples with CRP values above the upper limit of the calibration curve can be reanalyzed after automated or manual sample dilution.

We have developed a new procedure for turbidimetric measurement of ferritin concentration in human serum, based on latex microparticle agglutination technology. The most striking feature in Fig. The determination of ferritin is useful in diagnosis of defects in iron metabolism, monitoring iron therapy, ascertaining the iron reserves in groups at risk and in the differential diagnosis of anemias.

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It allows lower antibody coverage on the microparticle reagent and provides highly sensitive and robust particle-enhanced turbidimetric immunoassay for serum CRP.

Variability in the measurement of C-reactive protein in healthy subjects: Some use expensive monoclonal antibodies, and others use higher sample volumes and antibody concentrations in the reaction mixtures, which can increase nonspecific reactions We suggest an alternative approach that uses a small amount of immunopurified antibody and stabilizes the reagent by saturation of free surface with BSA in a two-step sequential covalent procedure.

The detection limits for CRP, defined as the mean plus 3 SD of the zero signal saline solutionwere 0.

Development of an automated immunoturbidimetric ferritin assay.

Clinical Chemistry Nov46 11. CRP is a prognostic risk factor for coronary heart disease in both patients with angina stable or not and in apparently healthy subjects 2and distinguishes rapidly destructive vs slowly progressive osteoarthritis 3. The effect of varying the particle size on a reagent with a constant charge of 0.

Ceffect of particle size on absorbance at nm. Development and validation of a particle enhanced turbidimetric imminoturbidimetric for C-reactive protein.

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Colloidal particles coated with polyclonal IgG usually are not very stable, mainly because of the high antibody coverage needed to obtain good sensitivities 3. The microparticle reagent response of the different antibodies to increasing CRP concentrations can be seen in Fig.

Development of an automated immunoturbidimetric ferritin assay.

Conditions for CRP determination. Research Article Technical Brief.

Download this assay page in pdf format. Ann Clin Biochem ; The microparticle reagents were immunoturhidimetric according to a previously published method by the carbodiimide [1-ethyl 3-dimethylamino-propyl carbodiimide chloride] procedure Jump to section Article Acknowledgments References.

Rev Rheum Engl Ed ; We describe a microparticle reagent that we use in an optimized turbidimetric procedure with immunopurified polyclonal antibodies against CRP.

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The covalently coupled isotherms of IgG anti-CRP on carboxyl-modified polystyrene particles nm diameter are shown in Fig. Ferritin determinations are widely used for monitoring renal anemia when iron utilization and distribution disorders are present during therapy with erythropoietin. J Immunol Methods ; Biomarker Menu Search Search by Keyword: Evaluation of an improved immunonephelometric assay ferrtin C-reactive protein [Abstract].

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